Molecular & Cellular Mycology - Workgroup Brosch

The main research focus of the lab lies on elucidating the functional impact of histone/protein arginine methylation on fungal physiology. We are interested in studying to what extent histone modifying activities are involved in fungal pathogenicity as well as to investigate their role in the regulation of secondary metabolite production. The elucidation of PRMT- and HDAC regulated bioactive molecules of Aspergillus is another issue.

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Topics

  • Chromatin-linked mechanisms of gene expression control
  • Isolation and identification of novel targets of PRMTs
  • PRMT- and HDAC regulated small bioactive molecules of Aspergillus

Technologies

  • Liquid chromatography (IEX, SEC, HIC, Affinity, RP) on preparative and analytical HPLC systems
  • In vivo and in vitro detection of chromatin modifiers (extraction of nuclear proteins, activity assays, autoradiography etc.)
  • Expression and purification of recombinant proteins in bacteria and fungi
  • Generation of mutant strains of filamentous fungi
  • Phenotypical and transcriptional analyses of (mutated) fungal strains

Key Publications

Bauer I, Graessle S, Loidl P, Hohenstein K, Brosch G (2010) Novel insights into the functional role of three protein arginine methyltransferases in Aspergillus nidulans. Fungal Genet Biol.; 47(6):551-61
Brosch G, Loidl P, Graessle S (2008) Histone modifications and chromatin dynamics: a focus on filamentous fungi. FEMS Microbiol Rev.;32(3):409-39.
Wang L, Kofler M, Brosch G, Melesina J, Sippl W, Martinez ED, Easmon J (2015) Benzazolyl-4-Piperazin-1-Ylsulfonylbenzenecarbohydroxamic Acids as Novel Selective Histone Deacetylase-6 Inhibitors with Antiproliferative Activity. PLoS One.; 10(12)

 

Group Leader

Assoz. Prof. Mag. Dr. Gerald Brosch

Medical University of Innsbruck

Division of Molecular Biology

Innrain 80-82/III, A 6020 Innsbruck

+43 512 9003 70211

This email address is being protected from spambots. You need JavaScript enabled to view it.

Group Members

  • Ingo Bauer, PhD

    PostDoc; Structure and function of chromatin: Histone and non-histone methylation in filamentous fungi Studies the impact of chromatin modifiers, in particular of PRMTs, on viability and metabolism of selected fungi. This email address is being protected from spambots. You need JavaScript enabled to view it.

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