Chromatin & Epigenetics

Histone acetylation is linked to transcriptional activation in eukaryotes and histone deacetylases (HDACs) are considered extraordinarily important for gene repression. Since some HDACs turned out to be essential for fungal pathogens or for the production of important fungal metabolites, we are analysing these enzymes as targets for novel antifungal agents or triggers of secondary metabolite (SM) production for the identification of novel bioactive molecules of fungi.

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Topics

  • Epigenetic gene regulation in filamentous fungi
  • Identification of complex partners and targets of HDACs in filamentous fungi
  • HDAC inhibitors as antifungal substances

Technologies

  • Generation of mutant strains of filamentous fungi
  • Phenotypical and transcriptional analyses of (mutated) fungal strains
  • Expression and/or purification of recombinant proteins or protein complexes in fungi
  • Chromatin immunoprecipitation (ChIP)
  • Activity assays of histone-modifying enzymes

Key Publications

Pidroni A, Faber B, Brosch G, Bauer I and Graessle S (2018). A Class 1 Histone Deacetylase as Major Regulator of Secondary Metabolite Production in Aspergillus nidulans. Front. Microbiol. 9:2212. doi: 10.3389/fmicb.2018.02212
Bauer, I., Varadarajan, D., Pidroni, A., Gross, S., Vergeiner, S., Faber, B., Hermann, M., Tribus, M., Brosch, G. and Graessle, S., (2016) A Class 1 Histone Deacetylase with Potential as Antifungal Target. mBio. 2016 Nov 1;7(6). pii: e00831-16. doi: 10.1128/mBio.00831-16
Brosch, G., Loidl, P. & Graessle, S. (2008). Histone Modifications and Chromatin Dynamics: A Focus on Filamentous Fungi. FEMS Microbiol Rev. 2008 May;32(3):409-39. doi: 10.1111/j.1574-6976.2007.00100.x. Review

 

Group Leader

Ao. Univ. Prof. Mag. Stefan Graessle

Medical University of Innsbruck

Division of Molecular Biology

Innrain 80-82/I, A 6020 Innsbruck

+43 512 9003 70201

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Group Members

  • Ingo Bauer, PhD

    PostDoc Impact of complex partners of histone deacetylases and role of arginine methyltransferases in filamentous fungi This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Angelo Pidroni, MSc

    PhD student Role of fungal-specific motifs in HDACs of filamentous fungi and the impact of these enzymes for the production of secondary metabolites (SM) This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Birgit Faber, MSc MSc

    PhD student The class 1 type HDAC HosA and its role in SM production and resistance to antifungal agents This email address is being protected from spambots. You need JavaScript enabled to view it.

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