Molecular & Cellular Mycology - Workgroup Brosch
The main research focus of the lab lies on elucidating the functional impact of histone/protein arginine methylation on fungal physiology. We are interested in studying to what extent histone modifying activities are involved in fungal pathogenicity as well as to investigate their role in the regulation of secondary metabolite production. The elucidation of PRMT- and HDAC regulated bioactive molecules of Aspergillus is another issue.
- Chromatin-linked mechanisms of gene expression control
- Isolation and identification of novel targets of PRMTs
- PRMT- and HDAC regulated small bioactive molecules of Aspergillus
- Liquid chromatography (IEX, SEC, HIC, Affinity, RP) on preparative and analytical HPLC systems
- In vivo and in vitro detection of chromatin modifiers (extraction of nuclear proteins, activity assays, autoradiography etc.)
- Expression and purification of recombinant proteins in bacteria and fungi
- Generation of mutant strains of filamentous fungi
- Phenotypical and transcriptional analyses of (mutated) fungal strains
Bauer I, Graessle S, Loidl P, Hohenstein K, Brosch G (2010) Novel insights into the functional role of three protein arginine methyltransferases in Aspergillus nidulans. Fungal Genet Biol.; 47(6):551-61
Brosch G, Loidl P, Graessle S (2008) Histone modifications and chromatin dynamics: a focus on filamentous fungi. FEMS Microbiol Rev.;32(3):409-39.
Wang L, Kofler M, Brosch G, Melesina J, Sippl W, Martinez ED, Easmon J (2015) Benzazolyl-4-Piperazin-1-Ylsulfonylbenzenecarbohydroxamic Acids as Novel Selective Histone Deacetylase-6 Inhibitors with Antiproliferative Activity. PLoS One.; 10(12)