Molecular Cell Biology of Filamentous Fungi

My research interest currently focusses on three main topics : (1) identification of molecular markers for mycoparasitism on the single cell level, (2) characterisation of host-specific stress responses that affect the polarised growth pattern of mycoparasitic Trichoderma species and ultimately define the outcome of the fungus-fungus interaction and (3) deciphering the key signal transduction pathways that regulate the attack-defence responses between mycoparasite and putative host fungus.

In addition, I have a constant interest in optimising live-cell imaging approaches for the visualisation and quantification of intracellular protein dynamics in various filamentous fungal species, including the well known model fungus Neurospora crassa, by using state-of-the-art microscopy systems in combination with vital fluorescent dyes and innovative fluorescent reporter constructs.

Alexander Lichius UIBK Profile

https://lfu.academia.edu/DrAlexanderLichius

 

 

Topics

  • Mycoparasitism & Fungal Stress Responses
  • Cell Polarity & Polarised Tip Growth
  • Live-Cell Imaging in Filamentous Fungi

Technologies

  • Fluorescent reporter technologies of target proteins with fluorescent proteins, fluorescent reporter molecules or organelle-specific vital fluorescent dyes
  • Molecular cloning & transformation for the generation of loss-of-function mutants by gene knock-out or targeted pharmacological inhibition of mutated alleles
  • Co-Immunoprecipitation & Mass Spectrometry for the isolation of protein-protein interaction partners and their identification
  • Live-cell imaging by means of confocal microscopy, deconvolution epifluorescence microscopy and widefield zoom microscopy
  • Biochemical approaches, such as process-specific inhibitors to interrogate target signalling or morphogenetic pathways

Key Publications

Atanasova L, Gruber S‡, Lichius A.‡, Radebner T, Abendstein L, Münsterkötter M, Stralis-Pavese N, Łabaj PP, Kreil DP, Zeilinger S. 2018 The Gpr1-regulated Sur7 family protein Sfp2 is required for hyphal growth and cell wall stability in the mycoparasite Trichoderma atroviride. Scientific Reports. 8(1). Nature
Lichius, A. ‡,*, A. Gorjachev, M.D. Fricker, B. Obara, E. Castro-Longoria and N.D. Read*. 2014. CDC-42 and RAC-1 regulate opposite chemotropism in Neurospora crassa. Journal of Cell Science 127:1953-1965. PubMed. “In This Issue” highlight: JCS
Lichius, A. ‡ and Yáñez-Gutiérrez, M.E. ‡, N.D. Read and E. Castro-Longoria. 2012. Comparative live-cell imaging analysis of SPA-2, BUD-6 and BNI-1 in Neurospora crassa revealed novel aspect of filamentous fungal polarisome architecture. PLoSOne 7(1):1-23. PLoSOne

 

Group Leader

Dr. Alexander Lichius

University of Innsbruck

Departement of Microbiology

Technikerstrasse 25, Victor-Franz-Hess Haus, 6th Floor, Room 6/04

+43 512 507 51256

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Group Members

  • BSc Laura Hackl, MSc student

    T. tip project – Analysis of the host-induced cell polarity stress in four different mycoparasitic Trichoderma species; Laura cloned and expressed species-specific CRIB biosensors in T. virens, T. asperellum and T. harzianum, and is now analysing changes in their recruitment dynamics upon host-induced cell polarity stress in comparison to T. atrovirideThis email address is being protected from spambots. You need JavaScript enabled to view it.
  • BSc Daniel Flatschacher, MSc student

    (co-supervised with Prof. Susanne Zeilinger) MicroSignalling project – Analysing Gpr1-Sfp2 interactions using GFP-trap based co-immunoprecipitation and mass spectrometry; Daniel established GFP-trap based co-immunoprecipitation procedures for the first time for T. atroviride and successfully showed by subsequent mass spectrometry that the plasma membrane proteins Gpr1 and Sfp2 interact physically. This email address is being protected from spambots. You need JavaScript enabled to view it.
  • MSc Dubraska Moreno-Ruiz, PhD student

    (co-supervised with Prof. Susanne Zeilinger) T. tip project – Analysing CRIB biosensor dynamics upon host-induced polarity stress in the context of MAPK signalling; Dubraska uses the CRIB biosensor in different MAPK gene deletion mutants to investigate changes in the polarity stress response upon host encounter. This email address is being protected from spambots. You need JavaScript enabled to view it.
  • MSc Leoni Abendstein (now pursuing a PhD at the University of Leiden, NL), MSc student

    (co-supervised with Prof. Susanne Zeilinger) MicroSignalling project – Analysing Gpr1-Sfp2 interactions using Bimolecular-Fluorescence-Complementation; Leoni established BiFC for the first time in T. atroviride and successfully showed that the plasma membrane proteins Gpr1 and Sfp2 interact physically in vivo. This email address is being protected from spambots. You need JavaScript enabled to view it.

Collaborators

  • Prof. Mark D. Fricker

    University of Oxford, Departement of Plant Sciences, South Parks Road Oxford, OX1 3RB, UK, This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Prof. Ernestina Castro-Longoria

    CICESE Carretera Ensenada - Tijuana No. 3918, Zona Playitas, CP. 22860, Ensenada, B.C. México. This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Prof. André Fleißner

    Technische Universität Braunschweig, Fakultät für Lebenswissenschaften, Institut für Genetik, Spielmannstraße 7, 38106 Braunschweig, This email address is being protected from spambots. You need JavaScript enabled to view it.