Master thesis project available at the Institute of Microbiology, working group Molecular Mycology and Mycoparasitism, in the context of developing new molecular tools to genetically manipulate the biocontrol fungus Trichoderma atroviride.
The start of the project would be end of May, but can be sooner if desired. Preliminary knowledge of basic genetic techniques is recommended.
The aim of the project will be to optimize inducible systems to Trichoderma species, test the tunability of the systems using a reporter protein and finally verify the system of choice by replacing the endogenous promoter of a gene of interest.
The methods/tasks of this thesis will include:
- PCRs/ gel electrophoresis
- Plasmid design and generation (assembly or Phusion PCR)
- Transformation of fungal protoplasts
- CRISPR/Cas9 mediated transformation of Trichoderma atroviride
- Fluorescence-based reporter assay (microscopy/plate reader)
If you are interested, please send your application to
We suggest a couple of days in our lab before starting the thesis to verify your interest in the project. We are looking forward to your application!
Web: https://www.uibk.ac.at/de/microbiology/forschung/mykologie/molekulare-mykologie/