Molecular Microbial Ecology

Working Group of Sabine Podmirseg

About

Ecology of anaerobic fungi (Neocallimastigomycota). Development of optimized tools for their detection, isolation and cultivation. Exploitation of their unique potential to degrade lignocellulosic material in order to improve anaerobic digestion processes. Investigating the microbial community associated with this peculiar microbial group. Investigation of different DNA fractions and their role in biomonitoring- and microbial activity surveys.

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- Molecular detection and quantification tools for anaerobic fungi
- Habitat distribution and microbial interactions of anaerobic fungi
- Molecular proxies for microbial activity (intracellular versus extracellular DNA)
- NGS (16S, 18S, 28S, ITS)
- qPCR
- Fluorescence microscopy (acid fuchsin staining)
- ADF/NDF fiber analysis
- FISH
→ Show all publications on PubMed

Projects

DACH HiPoAF (Lead) – Unleashing the hidden potential of anaerobic fungi (Neocallimastigomycota). The overarching aim of the proposed project that is being funded by the FWF with close to EUR 400,000 will be to remove existing methodological obstacles preventing the biotechnological utilization of anaerobic fungi. For that purpose, the consortium starts with the crucial basics, explores the cultivation requirements of Neocallimastigomycota, develops suitable culturing and detection methods and finally evaluates their biotechnological application for LCR disintegration.

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Spotlight on DNA extraction – extracellular DNA versus intracellular DNA versus total DNA and the information intrinsic to each DNA fraction.

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People

Group Leader

Mag. Dr. Sabine Podmirseg

University of Innsbruck - Departement of Microbiology
Sabine.Podmirseg@uibk.ac.at
+43 512 507 51321

Group Members

Julia Vinzelj, MSc

PhD Student, Detection and distribution of anaerobic fungi; HiPoAF; Julia.Vinzelj@uibk.ac.at

Magdalena Nagler, PhD

PostDoc – Anaerobic Fungi, intra- versus extracellular DNA; HiPoAF, in/exDNA project; magdalena.nagler@uibk.ac.at

Akshay Joshi, MSc

PhD Student - Cultivation and microbial interactions of anaerobic fungi; HiPoAF; aksh@zhaw.ch